New Analytical Method Development and Validation for the Estimation of Gemfibrozil in Bulk and Pharmaceutical Formulation by Rp-hplc
نویسندگان
چکیده
A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the determination Gemfibrozil in bulk and pharmaceutical dosage form. The column used was Agilent Zorbax C8 (150 ×4.6mm, 5μ) in gradient mode, with mobile phase containing Solvent-A: phosphate buffer adjusted the pH3.0 with orthophosphoric acid Solvent-B: Methanol the flow rate was 1.5 mL/ min and eluents was monitored at 276 nm. The retention time Gemfibrozil was 5.158 min, respectively. The linearity for Gemfibrozil was in the range of 30-450 μg/ml respectively. The recovery of Gemfibrozil was found to be 99.5%, respectively. The proposed method was validated and successfully applied to the estimation of Gemfibrozil in capsule dosage form.
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